F2502

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F2502

Aldrich

 

Fluorescein isothiocyanate isomer I

90%

Synonym:FITC, Fluorescein 5-isothiocyanate
CAS Number:3326-32-7
Linear Formula:C21H11NO5S
Molecular Weight:389.38
Beilstein Registry Number:1407295
EC Number:222-042-0
MDL number:MFCD00005063
PubChem Substance ID:24894823

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Description

ApplicationReagent for the FITC labeling of proteins; Microsequencing of proteins and peptides (HPLC)
Packaging1 g in glass btl
 50, 250 mg in glass btl
 Fluorescent labeling reagent for proteins.1,2,3,4,5 Used in the fluorescent antibody technique for rapid identification of pathogens.6,7

Properties

assay90%
fluorescenceλex 492 nm; λem 518 nm (green)
mp>360 °C(lit.)
UV absorptionλmax 499 nm

Safety

Personal Protective Equipmentdust mask type N95 (US), Eyeshields, Faceshields, Gloves
Hazard CodesXn
Risk Statements42/43
Safety Statements22-36/37-45
WGK Germany3

References

Cited Reference1. Nature 193, 167, (1962)
 2. Meth. Enzymol. 26, 28, (1972) Abstract
 3. Anal. Biochem. 57, 227, (1974) Abstract
 4. Proc. Natl. Acad. Sci. U. S. A. 72, 459, (1975) Abstract
 5. Fluorescent Protein Tracing Baltimore, MD , (1962)
 6. Am. J. Pathol. 34, 1081, (1958) Abstract
 7. Stain Technol. 44, 179, (1969) Abstract
referenceMiller, L., Protein modification of actin at lysine-61. Eur. J. Biochem. 174, 23, (1988)
 Der-Balian, G.P., FITC labelling of Fab'. Anal. Biochem. 173, 59, (1988)
 Wilderspin, A.F., Green, N.M., Modification of thiol groups. Anal. Biochem. 132, 449, (1983)
 Muramoto, K., Microsequencing of proteins and peptides (HPLC). Anal. Biochem. 141, 446, (1984)
 Aldrich MSDS 1, 948:C / Corp MSDS 1 (1), 1688:D / FT-IR 1 (2), 469:C / FT-IR 2 (3), 4153:C / FT-NMR 1 (2), 1560:B / IR-Spectra (2), 995:C / IR-Spectra (3), 1142:D / NMR-Reference 2 (2), 440:C / RegBook 1 (2), 2157:I / Sigma FT-IR 1 (2), 148:B / Stains and Dyes Ref, 377 / Structure Index 1, 445:C:5